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Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14528(B)] to Peroxiredoxin 5 - BSA and Azide free
  • Suitable for: ICC, WB, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free
    See all Peroxiredoxin 5 primary antibodies

  • Description

    Rabbit monoclonal [EPR14528(B)] to Peroxiredoxin 5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250136 is the carrier-free version of ab180123. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250136 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)

    This data was developed using ab180123, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HepG2 whole cell lysate (20 µg)

    Lane 4: A549 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab180123 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab180123 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. ab180123 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti-Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    All lanes : Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab180123, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    Immunocytochemistry - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    This data was developed using ab180123, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counterstained with DAPI (blue).
  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    This data was developed using ab180123, the same antibody clone in a different buffer formulation.Western Blot analysis of HeLa cell lysate immunoprecipitated with ab180123 at 1/60 dilution, using Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
  • Flow Cytometry - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    Flow Cytometry - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    This data was developed using ab180123, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/10 dilution (red) compared to a rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.
  • Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)
    Anti-Peroxiredoxin 5 antibody [EPR14528(B)] - BSA and Azide free (ab250136)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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