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Signal Transduction Cytoskeleton / ECM Extracellular Matrix

Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)

Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [RPSA/2699] to 67kDa Laminin Receptor - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, Protein Array, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free
    See all 67kDa Laminin Receptor primary antibodies
  • Description

    Mouse monoclonal [RPSA/2699] to 67kDa Laminin Receptor - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, Protein Array, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human 67kDa Laminin Receptor aa 1-295. The exact sequence is proprietary.
    Database link: P08865

  • Positive control

    • WB: THP-1, U937, SK-BR3, and A431 cell lysate. ICC/IF: HeLa and Raji cells. IHC-P: Human breast carcinoma, colon carcinoma, cervix carcinoma, basal cell carcinoma and tonsil tissue. Flow Cyt: Raji cells.
  • General notes

    ab268200 is the PBS only version of ab268079.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from bioreactor concentrate.
  • Clonality

    Monoclonal
  • Clone number

    RPSA/2699
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Extracellular Matrix
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Prions
    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina
    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)

    Formalin-fixed, paraffin-embedded human basal cell carcinoma tissue stained for 67kDa Laminin Receptor using ab268079 at 2 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079)

  • Immunocytochemistry/ Immunofluorescence - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
    Immunocytochemistry/ Immunofluorescence - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)

    Immunofluorescent staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for 67kDa Laminin Receptor using ab268079 at 2 µg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079)

  • Protein Array - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
    Protein Array - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)

    Protein Array containing more than 19,000 full-length human proteins using ab268079.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079).

  • Western blot - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
    Western blot - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
    All lanes : Anti-67kDa Laminin Receptor antibody [RPSA/2699] (ab268079) at 2 µg/ml

    Lane 1 : THP-1 (Human monocytic leukemia cell line) cell lysate
    Lane 2 : human U-937 cell lysate
    Lane 3 : SK-Br3 (Human mammary gland adenocarcinoma cell line) cell lysate
    Lane 4 : A431 (Human epidermoid carcinoma cell line) cell lysate

    Predicted band size: 33 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079)

  • Flow Cytometry - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
    Flow Cytometry - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)

    Flow Cytometric analysis of paraformaldehyde-fixed Raji (Human Burkitt's lymphoma cell line) cells labelling 67kDa Laminin Receptor using ab268079 at at 2 μg/ml followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079)

  • Immunocytochemistry/ Immunofluorescence - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
    Immunocytochemistry/ Immunofluorescence - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)

    Immunofluorescent staining of paraformaldehyde-fixed Raji (Human Burkitt's lymphoma cell line) cells stained for 67kDa Laminin Receptor using ab268079 at 2 µg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for 67kDa Laminin Receptor using ab268079 at 2 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)

    Formalin-fixed, paraffin-embedded human cervix carcinoma tissue stained for 67kDa Laminin Receptor using ab268079 at 2 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for 67kDa Laminin Receptor using ab268079 at 2 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for 67kDa Laminin Receptor using ab268079 at 2 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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