Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free (ab268200)
Key features and details
- Mouse monoclonal [RPSA/2699] to 67kDa Laminin Receptor - BSA and Azide free
- Suitable for: Flow Cyt, IHC-P, Protein Array, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-67kDa Laminin Receptor antibody [RPSA/2699] - BSA and Azide free
See all 67kDa Laminin Receptor primary antibodies -
Description
Mouse monoclonal [RPSA/2699] to 67kDa Laminin Receptor - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-P, Protein Array, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human 67kDa Laminin Receptor aa 1-295. The exact sequence is proprietary.
Database link: P08865 -
Positive control
- WB: THP-1, U937, SK-BR3, and A431 cell lysate. ICC/IF: HeLa and Raji cells. IHC-P: Human breast carcinoma, colon carcinoma, cervix carcinoma, basal cell carcinoma and tonsil tissue. Flow Cyt: Raji cells.
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General notes
ab268200 is the PBS only version of ab268079.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
RPSA/2699 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunofluorescent staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for 67kDa Laminin Receptor using ab268079 at 2 µg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079)
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Protein Array containing more than 19,000 full-length human proteins using ab268079.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079).
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All lanes : Anti-67kDa Laminin Receptor antibody [RPSA/2699] (ab268079) at 2 µg/ml
Lane 1 : THP-1 (Human monocytic leukemia cell line) cell lysate
Lane 2 : human U-937 cell lysate
Lane 3 : SK-Br3 (Human mammary gland adenocarcinoma cell line) cell lysate
Lane 4 : A431 (Human epidermoid carcinoma cell line) cell lysate
Predicted band size: 33 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079)
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Flow Cytometric analysis of paraformaldehyde-fixed Raji (Human Burkitt's lymphoma cell line) cells labelling 67kDa Laminin Receptor using ab268079 at at 2 μg/ml followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079)
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Immunofluorescent staining of paraformaldehyde-fixed Raji (Human Burkitt's lymphoma cell line) cells stained for 67kDa Laminin Receptor using ab268079 at 2 µg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268079)