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Anti-Laminin 5 antibody [P3H9-2] - BSA and Azide free (ab174717)

Key features and details

  • Mouse monoclonal [P3H9-2] to Laminin 5 - BSA and Azide free
  • Suitable for: IHC-Fr
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Laminin 5 antibody [P3H9-2] - BSA and Azide free
    See all Laminin 5 primary antibodies

  • Description

    Mouse monoclonal [P3H9-2] to Laminin 5 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Human Laminin 5. Specifically, human keratinocytes.

  • Positive control

    • IHC-Fr: Normal human colon and skin frozen sections.

  • General notes

    This antibody clone is manufactured by Abcam.

    This product is the PBS-only buffer version of ab78286.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Laminin 5 antibody [P3H9-2] - BSA and Azide free (ab174717)
    Immunohistochemistry (Frozen sections) - Anti-Laminin 5 antibody [P3H9-2] - BSA and Azide free (ab174717)

    This IHC data was generated using the same antibody clone in a different buffer formulation (ab78286). 

    IHC image of Laminin 5 staining in a section of frozen normal human colon*, performed on a Leica Bond™ system using the standard protocol F. No antigen retrieval step was performed prior to staining. The section was incubated with ab78286, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Immunohistochemistry (Frozen sections) - Anti-Laminin 5 antibody [P3H9-2] - BSA and Azide free (ab174717)
    Immunohistochemistry (Frozen sections) - Anti-Laminin 5 antibody [P3H9-2] - BSA and Azide free (ab174717)

    This IHC data was generated using the same antibody clone in a different buffer formulation (ab78286). 

    IHC image of Laminin 5 staining in a section of frozen normal human skin*, performed on a Leica Bond™ system using the standard protocol F. No antigen retrieval step was performed prior to staining. The section was incubated with ab78286, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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