Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Laminin beta 2 antibody [EPR23568-91] - BSA and Azide free (ab277530)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23568-91] to Laminin beta 2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Laminin beta 2 antibody [EPR23568-91] - BSA and Azide free
    See all Laminin beta 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23568-91] to Laminin beta 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human heart, colon, skeletal muscle and placenta tissue lysates; mouse heart tissue lysate; rat heart tissue lysate.

  • General notes

    ab277530 is the carrier-free version of ab277521. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277530 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Laminin beta 2 antibody [EPR23568-91] - BSA and Azide free (ab277530)
    Western blot - Anti-Laminin beta 2 antibody [EPR23568-91] - BSA and Azide free (ab277530)
    All lanes : Anti-Laminin beta 2 antibody [EPR23568-91] (ab277521) at 1/1000 dilution

    Lane 1 : Human heart tissue lysate
    Lane 2 : Human colon tissue lysate
    Lane 3 : Human skeletal muscle tissue lysate
    Lane 4 : Human placenta tissue lysate
    Lane 5 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 196 kDa
    Observed band size: 200 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab277521, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 9273822 ).

    Low expression: human liver (PMID:7795887).

    Exposure time: Lane 1: 48 seconds

    Lane 2-5: 3minutes.

  • Western blot - Anti-Laminin beta 2 antibody [EPR23568-91] - BSA and Azide free (ab277530)
    Western blot - Anti-Laminin beta 2 antibody [EPR23568-91] - BSA and Azide free (ab277530)
    All lanes : Anti-Laminin beta 2 antibody [EPR23568-91] (ab277521) at 1/1000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat heart tissue lysate
    Lane 4 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 196 kDa
    Observed band size: 200 kDa why is the actual band size different from the predicted?



    This data was developed using ab277521, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 9273822). 

    Low expression: mouse liver, rat liver (PMID: 7795887)

    Exposure time: Lane 1, 2, 4: 3minutes

    Lane 3: 70 seconds.

  • Anti-Laminin beta 2 antibody [EPR23568-91] - BSA and Azide free (ab277530)
    Anti-Laminin beta 2 antibody [EPR23568-91] - BSA and Azide free (ab277530)

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