Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Rabbit monoclonal [EP557Y] to MKK6
Suitable for: WB, IP
Knockout validated
Reacts with: Mouse, Rat, Human
Isotype: IgG

Product name

Anti-MKK6 antibody [EP557Y]
See all MKK6 primary antibodies
Description

Rabbit monoclonal [EP557Y] to MKK6
Host species

Rabbit
Specificity

ab33866 recognises MKK6.
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human MKK6 aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- WB: HepG2, C2C12, HeLa, HAP1, and C6 lysates IP: HepG2 cells
General notes
This product was previously labelled as MEK6.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP557Y
Isotype

IgG
Research areas

Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)

Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: MEK6 knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
Lanes 4, 8 and 12: A431 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target – ab33866 observed at 37 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control – ab18058 observed at 130 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signal

ab33866 (unpurified) was shown to specifically react with MEK6 when MEK6 knockout samples were used. Wild-type and MEK6 knockout samples were subjected to SDS-PAGE. ab33866 and ab18058 (loading control to Vinculin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)

All lanes : Anti-MKK6 antibody [EP557Y] (ab33866) at 1/1000 dilution

Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 2 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 37 kDa
Observed band size: 36 kDa
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-MKK6 antibody [EP557Y] (ab33866)

ab33866 (purified) at 1/20 dilution (1 µg) immunoprecipitating MKK6 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+): ab33866 & HepG2 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab33866 in HepG2 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.

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