Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MKK6 antibody [EP557Y] (ab33866)

Key features and details

  • Rabbit monoclonal [EP557Y] to MKK6
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-MKK6 antibody [EP557Y]
    See all MKK6 primary antibodies

  • Description

    Rabbit monoclonal [EP557Y] to MKK6

  • Host species

    Rabbit

  • Specificity

    ab33866 recognises MKK6.

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MKK6 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HepG2, C2C12, HeLa, HAP1, and C6 lysates IP: HepG2 cells

  • General notes

    This product was previously labelled as MEK6.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)
    Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)

    Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
    Lanes 2, 6 and 10: MEK6 knockout HAP1 cell lysate (20 µg)
    Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
    Lanes 4, 8 and 12: A431 cell lysate (20 µg)
    Lanes 1, 2, 3 and 4: Green signal from target – ab33866 observed at 37 kDa
    Lanes 5, 6, 7 and 8: Red signal from loading control – ab18058 observed at 130 kDa
    Lanes 9, 10, 11 and 12: Merged (red and green) signal

    ab33866 (unpurified) was shown to specifically react with MEK6 when MEK6 knockout samples were used. Wild-type and MEK6 knockout samples were subjected to SDS-PAGE. ab33866 and ab18058 (loading control to Vinculin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)
    Western blot - Anti-MKK6 antibody [EP557Y] (ab33866)
    All lanes : Anti-MKK6 antibody [EP557Y] (ab33866) at 1/1000 dilution

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 2 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
    Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-MKK6 antibody [EP557Y] (ab33866)
    Immunoprecipitation - Anti-MKK6 antibody [EP557Y] (ab33866)
    ab33866 (purified) at 1/20 dilution (1 µg) immunoprecipitating MKK6 in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab33866 & HepG2 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab33866 in HepG2 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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