Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MKK6 antibody [EP557Y] - BSA and Azide free (ab269878)

Key features and details

  • Rabbit monoclonal [EP557Y] to MKK6 - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-MKK6 antibody [EP557Y] - BSA and Azide free
    See all MKK6 primary antibodies

  • Description

    Rabbit monoclonal [EP557Y] to MKK6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, C2C12, HeLa, HAP1, and C6 lysates. IP: HepG2 cell lysate.

  • General notes

    This product was previously labelled as MEK6.

    ab269878 is the carrier-free version of ab33866. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-MKK6 antibody [EP557Y] (ab269878)
    Western blot - Anti-MKK6 antibody [EP557Y] (ab269878)

    Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
    Lanes 2, 6 and 10: MEK6 knockout HAP1 cell lysate (20 µg)
    Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
    Lanes 4, 8 and 12: A431 cell lysate (20 µg)
    Lanes 1, 2, 3 and 4: Green signal from target – ab33866 observed at 37 kDa
    Lanes 5, 6, 7 and 8: Red signal from loading control – ab18058 observed at 130 kDa
    Lanes 9, 10, 11 and 12: Merged (red and green) signal

    ab33866 (unpurified) was shown to specifically react with MEK6 when MEK6 knockout samples were used. Wild-type and MEK6 knockout samples were subjected to SDS-PAGE. ab33866 and ab18058 (loading control to Vinculin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab33866).

  • Immunoprecipitation - Anti-MKK6 antibody [EP557Y] (ab269878)
    Immunoprecipitation - Anti-MKK6 antibody [EP557Y] (ab269878)

    ab33866 (purified) at 1/20 dilution (1 µg) immunoprecipitating MKK6 in HepG2 whole cell lysate. 
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10 µg 
    Lane 2 (+): ab33866 & HepG2 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab33866 in HepG2 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab33866).

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