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Native human Cathepsin B protein (ab90387)

Key features and details

  • Expression system: Native
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE, WB

Description

  • Product name

    Native human Cathepsin B protein
    See all Cathepsin B proteins and peptides

  • Biological activity

    Activity: Greater than 200 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one µmole of Z-Arg-Arg-beta-naphthylamide per minute at 40°C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Native

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Native

    • Species

      Human

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 5.00
    Constituents: 0.41% Sodium acetate, 0.0292% EDTA

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

  • Western blot - Native human Cathepsin B protein (ab90387)
    Western blot - Native human Cathepsin B protein (ab90387)
    Anti-Cathepsin B antibody [CA10] (ab58802) at 0.25 µg/ml + Native human Cathepsin B protein (ab90387) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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