Mouse Fetuin A Matched Antibody Pair Kit (ab219522)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 312.5 pg/ml - 20000 pg/ml
Overview
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Product name
Mouse Fetuin A Matched Antibody Pair Kit
See all Fetuin A kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
128.4 pg/ml -
Range
312.5 pg/ml - 20000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Fetuin A Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Fetuin A.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab204526. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Fetuin A Capture Antibody 2 x 50µg 1 x 50µg Mouse Fetuin A Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Fetuin A Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. -
Tissue specificity
Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. -
Sequence similarities
Belongs to the fetuin family.
Contains 2 cystatin fetuin-A-type domains. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 59 kDa bone sialic acid containing protein
- A2HS
- Aa2 066
see all -
Database links
- Entrez Gene: 11625 Mouse
- SwissProt: P29699 Mouse
- Unigene: 197554 Mouse
Images
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Standard calibration curve. Background subtracted values are graphed.
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To learn more about the advantages of recombinant antibodies see here.