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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins

Mouse Fetuin A Matched Antibody Pair Kit (ab219522)

Mouse Fetuin A Matched Antibody Pair Kit (ab219522)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Mouse
  • Range: 312.5 pg/ml - 20000 pg/ml

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Overview

  • Product name

    Mouse Fetuin A Matched Antibody Pair Kit
    See all Fetuin A kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    128.4 pg/ml
  • Range

    312.5 pg/ml - 20000 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse Fetuin A Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Fetuin A.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab204526. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Mouse Fetuin A Capture Antibody 2 x 50µg 1 x 50µg
    Mouse Fetuin A Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Mouse Fetuin A Lyophilized Protein 2 vials 1 vial
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Metabolism
    • Types of disease
    • Obesity
  • Function

    Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.
  • Tissue specificity

    Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
  • Sequence similarities

    Belongs to the fetuin family.
    Contains 2 cystatin fetuin-A-type domains.
  • Post-translational
    modifications

    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02765 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 59 kDa bone sialic acid containing protein
    • A2HS
    • Aa2 066
    • AHS
    • Ahsg
    • Alpha 2 HS glycoprotein
    • Alpha 2 HS glycoprotein chain A
    • Alpha 2 HS glycoprotein chain B
    • Alpha 2 Z globulin
    • Alpha 2HS glycoprotein
    • Alpha-2-HS-glycoprotein chain B
    • Alpha-2-Z-globulin
    • alpha2 Heremans Schmid glycoprotein
    • Asialofetuin
    • Ba alpha 2 glycoprotein
    • Ba-alpha-2-glycoprotein
    • BSP
    • Countertrypin
    • FETUA
    • FETUA_HUMAN
    • Fetuin A
    • Fetuin, mouse, homolog of
    • Fetuin-A
    • FetuinA
    • Glycoprotein PP63
    • HSGA
    • pp63
    see all
  • Database links

    • Entrez Gene: 11625 Mouse
    • SwissProt: P29699 Mouse
    • Unigene: 197554 Mouse

    Images

    • Mouse Fetuin A standard curve
      Mouse Fetuin A standard curve

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Mouse Fetuin A Matched Antibody Pair Kit (ab219522)
      Sandwich ELISA - Mouse Fetuin A Matched Antibody Pair Kit (ab219522)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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