Anti-Mob1A antibody (ab236969)
Key features and details
- Rabbit polyclonal to Mob1A
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Mob1A antibody
See all Mob1A primary antibodies -
Description
Rabbit polyclonal to Mob1A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Orangutan
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Immunogen
Recombinant full length protein corresponding to Human Mob1A aa 1-250.
Database link: Q9H8S9 -
Positive control
- WB: Jurkat and K562 whole cell lysate; Mouse small intestine tissue lysate. IHC-P: Human small intestine and breast cancer tissue. ICC/IF: HepG2 cells. IP: Jurkat whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Mob1A antibody (ab236969)All lanes : Anti-Mob1A antibody (ab236969) at 1/200 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Mouse small intestine tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mob1A antibody (ab236969)Paraffin-embedded human small intestine tissue stained for Mob1A using ab236969 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Mob1A antibody (ab236969)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Mob1A (Green) using ab236969 at 1/333 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
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Western blot - Anti-Mob1A antibody (ab236969)Anti-Mob1A antibody (ab236969) at 1/200 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
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Immunoprecipitation - Anti-Mob1A antibody (ab236969)Mob1A was immunoprecipitated from 500 μg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 1: Rabbit control IgG instead of ab236969 in Jurkat cell lysate.
Lane 2: ab236969 IP in Jurkat cell lysate.
Lane 3: Jurkat whole cell lysate (input).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mob1A antibody (ab236969)Paraffin-embedded human breast cancer tissue stained for Mob1A using ab236969 at 1/1000 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
