Recombinant human Cathepsin B protein (ab198475)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Cathepsin B protein
See all Cathepsin B proteins and peptides -
Biological activity
Specific Activity: ≥ 4048 pmol/min/µg
Assay condition: 25 mM MES, pH 5.0, 10 μM substrate (Z-Leu-Arg-AMC), 10 min, room temperature. Prior to assay enzyme was activated with preincubation at 10 μg/ml in 25 mM MES, pH 5.0, 5 mM DTT for 15 min at RT.
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Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MPLLLLLPLLWAGALARSRPSFHPLSDELVNYVNKRNTTWQAGHNFYNVD MSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRD QGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCN GGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGE GDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEG AFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWN TDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKI -
Predicted molecular weight
43 kDa including tags -
Amino acids
18 to 339 -
Tags
His tag C-Terminus -
Additional sequence information
This product is the mature full length protein from aa 18 to 339. The signal peptide is not included, but it contains CD33 signal peptide (a.a. 1-16) instead.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.36% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human Cathepsin B protein (ab198475)
10% SDS Page analysis of ab198475 with Coomassie staining.
Lane 1: 7 μg ab198475
Lane 2: Protein marker -
Functional Studies - Recombinant human Cathepsin B protein (ab198475)Example of specific activity of ab198475.
