Anti-CD44 antibody [M010] - Extracellular domain (ab253276)
![Anti-CD44 antibody [M010] - Extracellular domain (ab253276)](https://www.abcam.com/ps/products/253/ab253276/Images/ab253276-344288-AntiCD44-antibody-M010--Extracellular-domain-immunocytochemistry-A431-human.jpg "Anti-CD44 antibody [M010] - Extracellular domain (ab253276)")
Key features and details
- Mouse monoclonal [M010] to CD44 - Extracellular domain
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-CD44 antibody [M010] - Extracellular domain
See all CD44 primary antibodies -
Description
Mouse monoclonal [M010] to CD44 - Extracellular domain -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type within Human CD44 (extracellular). The exact sequence is proprietary. From the A431 cell line.
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Positive control
- WB: A549 and MDA-MB-231 cell lysate. ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
M010 -
Isotype
IgG2b -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [M010] - Extracellular domain (ab253276)
Paraformaldehyde-fixed A431 (Human epidermoid carcinoma cell line) cells labeling CD44 using ab253276 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.
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![Western blot - Anti-CD44 antibody [M010] - Extracellular domain (ab253276)](https://www.abcam.com/ps/products/253/ab253276/Images/ab253276-344287-AntiCD44-antibody-M010--Extracellular-domain-western-blot-MDAMB231-A549-human.jpg)
Western blot - Anti-CD44 antibody [M010] - Extracellular domain (ab253276)All lanes : Anti-CD44 antibody [M010] - Extracellular domain (ab253276) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate, native conditions
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate, denatured conditions
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate, native conditions
Lane 4 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate, denatured conditionsWestern blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hr at room temperature.