Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CBR1 antibody [EPR9660] (ab156590)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9660] to CBR1
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CBR1 antibody [EPR9660]
    See all CBR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9660] to CBR1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide, corresponding to residues in Human CBR1 (Uniprot: P16152).

  • Positive control

    • HeLa, 293T, SH-SY5Y and Human fetal kidney lysates; Human kidney tissue; HeLa cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CBR1 antibody [EPR9660] (ab156590)
    Western blot - Anti-CBR1 antibody [EPR9660] (ab156590)
    All lanes : Anti-CBR1 antibody [EPR9660] (ab156590) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : CBR1 knockout A549 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab156590 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab156590 Anti-CBR1 antibody [EPR9660] was shown to specifically react with CBR1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266954 (knockout cell lysate ab257874) was used. Wild-type and CBR1 knockout samples were subjected to SDS-PAGE. ab156590 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)

    ab156590 showing +ve staining in Human thyroid gland carcinoma.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CBR1 antibody [EPR9660] (ab156590)
    Immunocytochemistry/ Immunofluorescence - Anti-CBR1 antibody [EPR9660] (ab156590)
    Immunofluorescent analysis of HeLa cells labeling CBR1 with ab156590 at 1/50 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)

    ab156590 showing +ve staining in Human skeletal muscle.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)

    ab156590 showing +ve staining in Human gastric carcinoma.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CBR1 with ab156590 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-CBR1 antibody [EPR9660] (ab156590)
    Western blot - Anti-CBR1 antibody [EPR9660] (ab156590)
    All lanes : Anti-CBR1 antibody [EPR9660] (ab156590) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 30 kDa

  • Anti-CBR1 antibody [EPR9660] (ab156590)
    Anti-CBR1 antibody [EPR9660] (ab156590)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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