Cobalt Assay Kit (ab102510)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.18 µg
Overview
-
Product name
Cobalt Assay Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 0.18 µg -
Range
0.18 µg - 3 µg -
Assay time
0h 20m -
Product overview
Abcam's Cobalt Assay Kit takes advantage of the reaction of Co2+ with mercaptoethanol in basic media. Under the condition, Cobalt forms a complex with a strong absorbance band at 475 nm. The metal ions Fe2+, Cu2+, Ni2+, Zn2+, Mn2+, exhibit less than 10% interference at this wavelength. The kit provides a quick simple accurate method of quantitating Co2+ in a variety of samples.
Visit our FAQs page for tips and troubleshooting. -
Notes
Cobalt is a hard, lustrous, grey metal, a chemical element (symbol Co, atomic number 27 and atomic mass 58.9). Cobalt is an essential trace-element for all multicellular organisms as the active center of coenzymes called cobalamins. These include vitamin B-12 which is essential for plants and animals. Cobalt is also an active nutrient for bacteria, algae, and fungi, and may be a necessary nutrient for all life. Cobalt, particularly the Co2+ species, forms complexes with a large number of organic and inorganic molecules with a range of extinction coefficients in the visible range.
-
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Cobalt Chloride Standard Yellow 1 unit Cobalt Reagent Red 1 x 1ml -
Research areas
-
Relevance
Cobalt is a hard, lustrous, grey metal, a chemical element (symbol Co, atomic number 27 and atomic mass 58.9). Cobalt is an essential trace-element for all multicellular organisms as the active center of coenzymes called cobalamins. These include vitamin B-12 which is essential for plants and animals. Cobalt is also an active nutrient for bacteria, algae, and fungi, and may be a necessary nutrient for all life. Cobalt, particularly the Co+2 species, forms complexes with a large number of organic and inorganic molecules with a range of extinction coefficients in the visible range. -
Alternative names
- Co
Images
-
Functional Studies - Cobalt Assay Kit (ab102510)Cobalt Standard Curve: Assays were performed following the kit protocol.
