Anti-HMBS/PBGD antibody (ab232927)
Key features and details
- Rabbit polyclonal to HMBS/PBGD
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-HMBS/PBGD antibody
See all HMBS/PBGD primary antibodies -
Description
Rabbit polyclonal to HMBS/PBGD -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow
-
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human HMBS/PBGD aa 50-350. (Expressed in E.coli).
Database link: P08397 -
Positive control
- WB: Recombinant human HMBS/PBGD protein; HeLa, HEK-293T and HepG2 cell lysates; Pig heart lysate. IHC-P: Human pancreatic cancer tissue. ICC/IF: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab232927 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-HMBS/PBGD antibody (ab232927)Anti-HMBS/PBGD antibody (ab232927) at 2 µg/ml + Recombinant human HMBS/PBGD protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 39 kDa
-
Western blot - Anti-HMBS/PBGD antibody (ab232927)All lanes : Anti-HMBS/PBGD antibody (ab232927) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 4 : Pig heart lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 39 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMBS/PBGD antibody (ab232927)Paraffin-embedded human pancreatic cancer tissue stained for HMBS/PBGD using ab232927 at 10 μg/ml in immunohistochemical analysis. DAB staining.
-
Immunocytochemistry/ Immunofluorescence - Anti-HMBS/PBGD antibody (ab232927)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HMBS/PBGD (green) using ab232927 at 20 μg/ml in ICC/IF, followed by FITC staining.
