Anti-HMBS/PBGD antibody (ab185929)
Key features and details
- Rabbit polyclonal to HMBS/PBGD
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HMBS/PBGD antibody
See all HMBS/PBGD primary antibodies -
Description
Rabbit polyclonal to HMBS/PBGD -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human HMBS/PBGD aa 300 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_000181.2
Database link: P08397 -
Positive control
- HeLa, 293T and Jurkat cell lysates
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General notes
This product was previously labelled as HMBS
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab185929 is affinity purified using an epitope specific to HMBS/PBGD immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-HMBS/PBGD antibody (ab185929)
Western blot analysis of Immunoprecipitates.
Immunoprecipitation of 293T whole cell lysate (1 mg for IP; 20% of IP loaded) using ab185929 at 6 µg/mg lysate for IP and at 1 µg/ml for blotting
Detection: Chemiluminescence with an exposure time of 10 seconds.
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Western blot - Anti-HMBS/PBGD antibody (ab185929)All lanes : Anti-HMBS/PBGD antibody (ab185929) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 39 kDa
Exposure time: 3 minutes
