Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-beta Casein antibody [EPR12179] - BSA and Azide free (ab232662)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12179] to beta Casein - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-beta Casein antibody [EPR12179] - BSA and Azide free
    See all beta Casein primary antibodies

  • Description

    Rabbit monoclonal [EPR12179] to beta Casein - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human milk lysate.

  • General notes

    Ab232662 is the carrier-free version of ab205301. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232662 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-beta Casein antibody [EPR12179] - BSA and Azide free (ab232662)
    Immunoprecipitation - Anti-beta Casein antibody [EPR12179] - BSA and Azide free (ab232662)

    beta Casein was immunoprecipitated from 1mg of Human milk lysate with ab205301 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab205301 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution.

    Lane 1: Human milk lysate10 µg (Input). Lane 2: ab205301 IP in Human milk lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205301 in Human milk lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205301).

  • Western blot - Anti-beta Casein antibody [EPR12179] - BSA and Azide free (ab232662)
    Western blot - Anti-beta Casein antibody [EPR12179] - BSA and Azide free (ab232662)
    All lanes : Anti-beta Casein antibody [EPR12179] (ab205301) at 1/1000 dilution

    Lane 1 : Human milk lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 25 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205301).

  • Anti-beta Casein antibody [EPR12179] - BSA and Azide free (ab232662)
    Anti-beta Casein antibody [EPR12179] - BSA and Azide free (ab232662)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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