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Human Siglec 7 ELISA Kit (ab267615)

Key features and details

  • Sensitivity: 0.1 ng/ml
  • Range: 0.102 ng/ml - 25 ng/ml
  • Sample type: Cell culture media, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Siglec 7 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Serum, Plasma, Cell culture media

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.1 ng/ml

  • Range

    0.102 ng/ml - 25 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 82 70% - 97%
    Plasma 78 68% - 93%
    Cell culture media 141 132% - 147%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Siglec 7 ELISA Kit is designed for the quantitative determination of Siglec 7 in cell culture supernatants, plasma and serum samples.


    This assay employs an antibody specific for human Siglec 7 coated on a 96-well plate. Standards and samples are pipetted into the wells and Siglec 7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Siglec 7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Siglec 7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS13, Aggrecan, Angiotensinogen (SERPINA8), B7-H1 (CD274), BMPR-IA (ALK-3), BMPR-II, Cadherin-11, CD27 (TNFRSF7), CD6, Ck beta 8-1 (CCL23), CNTF, DNAM-1 (CD226), CD147 (EMMPRIN), FLRG, Follistatin-like 1, Fractalkine (CX3CL1), Galectin-1, GITR Ligand (TNFSF18), Granulysin (NKG5/LAG-2), IL-1 R3 (IL-1 R Acp), IL-15 R alpha, IL-17E (IL-25), IL32 alpha, CHL-1 (L1CAM-2), LRIG3, LRP-6, MEPE (OF45), Nectin-4, Periostin, Persephin, Renin, RGM-B, ROBO3, S100 A8, Syndecan-3, Thrombospondin 2, Thrombospondin 5 (COMP), Tie-1, ULBP-2 (ALCAN-alpha/N2DL2).

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    5X Assay Diluent D 1 x 15ml
    Anti-Human Siglec 7 coated Microplate (12 x 8 wells) 1 unit
    Biotinylated Anti-Human Siglec 7 Detection Antibody 2 vials
    Human Siglec 7 Standard (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Solution 1 x 12ml

  • Research areas

  • Function

    Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).

  • Tissue specificity

    Predominantly expressed by resting and activated natural killer cells and at lower levels by granulocytes and monocytes. High expression found in placenta, liver, lung, spleen, and peripheral blood leukocytes.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Domain

    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

  • Post-translational
    modifications

    Tyrosine phosphorylated.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • Adhesion inhibitory receptor molecule 1
    • Adhesion inhibitory receptor molecule 1, siglec 7
    • AIRM 1
    • AIRM-1
    • AIRM1
    • CD328
    • CD328 antigen
    • CDw328
    • D siglec
    • D-siglec
    • HLDA 8
    • p75
    • p75/AIRM1
    • QA79
    • QA79 membrane protein
    • Sialic acid binding Ig like lectin 7
    • Sialic acid binding immunoglobulin like lectin 7
    • Sialic acid-binding Ig-like lectin 7
    • SIGL7_HUMAN
    • Siglec-7
    • SIGLEC19P
    • SIGLEC7
    • SIGLECP2
    see all

  • Database links

    Images

    • Standard curve
      Standard curve

      Human Siglec 7 ELISA kit (ab267615) Standard curve.

      Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

       

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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