Anti-CBR1 antibody (ab4148) at 0.03 µg/ml + Human liver lysate at 35 µg

Predicted band size: 30 kDa

Primary incubation 1 hour at room temperature. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence analysis of U251 cells labelling CBR1 with ab4148 at 10 µg/mL. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody incubation was for 1 hour followed by incubation with an Alexa Fluor^® 488-conjugated secondary antibody at 2 µg/mL. DAPI nuclear counterstain was used.

Negative control: Unimmunized goat IgG (10 µg/mL) followed by an Alexa Fluor^® 488-conjugated secondary antibody (2 µg/mL).

ab4148 (3ug/ml) staining human CBR1 in human kidney by immunohistochemistry using paraffin embedded tissue.

Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Lanes 1 & 10 : Protein Marker
Lanes 2-9 : Anti-CBR1 antibody (ab4148) at 1/1000 dilution (in PBS + (0.05%) Tween at 25°C )

Lanes 1 & 10 : Protein Marker
Lanes 2-5 : Whole cell lysate of human lung cancer cell line A549 at 25 µg
Lane 6 : 1X SDS Loading Buffer
Lane 7 : recombinant CBR1 at 0.03 µg
Lane 8 : recombinant CBR1 at 0.07 µg
Lane 9 : recombinant CBR1 at 0.1 µg

Secondary
Lanes 2-9 : An HRP-conjugated anti-goat IgG at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 30 kDa
Observed band size: 30 kDa


Exposure time: 1 minute


Blocking Step: 5% Milk for 1 hour at 25°C