Hydrogen Peroxide Assay Kit (Cell-based) (ab138874)
Key features and details
- Assay type: Cell-based
- Detection method: Fluorescent
- Platform: Fluorescence microscope
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells
- Sensitivity: 3 µM
Overview
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Product name
Hydrogen Peroxide Assay Kit (Cell-based)
See all Hydrogen Peroxide kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Sensitivity
3 µM -
Assay time
0h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Hydrogen Peroxide Assay Kit (Cell-based) (ab138874) uses our AbGreen Indicator to quantify hydrogen peroxide in live cells. The indicator is cell-permeable, and generates green fluorescence when it reacts with hydrogen peroxide.
The assay is in a “mix and read” assay format that is compatible with HTS liquid handling instruments and a 96-well or 384-well plate format. It provides a sensitive, one-step fluorometric assay to detect as little as 0.3 nanomoles of H2O2 in a 100 µL assay volume (3 µM). Results can be read by either a fluorescence microplate reader at Ex/Em = 490/520 nm for H2O2 detection in solution or a fluorescence microscopy for live cell H2O2 imaging.
Hydrogen peroxide assay protocol summary:
- add samples and standards to wells
- add green indicator solution
- analyze with microplate reader for 15-60 min.
Alternatively, for live cell assay:
- add green indicator solution to cells
- incubate for 5-60 min whilst analyzing with microplate reader or fluorescence microscope -
Notes
Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. It is involved in many biological events that are linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down’s syndrome. The measurement of this reactive species is helpful for determining how oxidative stress modulates various intracellular pathways.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests AbGreen Indicator 1 vial Assay Buffer 1 x 20ml DMSO 1 x 200µl Hydrogen Peroxide Solution 1 x 200µl -
Research areas
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Relevance
Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome and immune system diseases. -
Alternative names
- H2O2
Images
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Staining of HeLa cells using Hydrogen Peroxide Assay Kit (Cell-based) (ab138874)
Fluorescence images of intracellular hydrogen peroxide in HeLa cells using Hydrogen Peroxide Assay Kit (Cell-based) (ab138874). HeLa cells were treated with (left) or without (right) 100 µM hydrogen peroxide at 37ºC for 90 minutes.
