Hydrogen Peroxide Assay Kit (ab102500)
Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.04 µM
Overview
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Product name
Hydrogen Peroxide Assay Kit
See all Hydrogen Peroxide kits -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Quantitative -
Sensitivity
> 0.04 µM -
Assay time
1h 00m -
Product overview
Hydrogen Peroxide Assay Kit ab102500 is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H2O2 in biological samples.
In the hydrogen peroxide assay protocol, horse radish peroxidase (HRP) reacts with a probe and H2O2 to produce a product with color (λmax = 570 nm) and red-fluorescence (Ex/Em=535/587 nm).
The detection limit is 2 pmol per assay (or 40 nM concentration) of H2O2 in the sensitive fluorometric assay.
Hydrogen peroxide assay protocol summary:
- add standards and samples to wells
- add reaction mix and incubate for 10 min
- analyze with microplate reader -
Notes
The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method.
Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kappaB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down's syndrome and immune system diseases.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 200 tests H2O2 Assay Buffer WM 1 x 25ml H2O2 Standard Yellow 1 x 0.1ml HRP Green 1 unit OxiRed Probe Red 1 x 200µl -
Research areas
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Relevance
Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome and immune system diseases. -
Alternative names
- H2O2
Images
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Functional Studies - Hydrogen Peroxide Assay Kit (ab102500) Vicente, Cl?udia S L et al., PloS one?vol. 10,4 e0123839., Fig 2, doi:10.1371/journal.pone.0123839
H2O2 neutralization of of Bursaphelenchus sp. isolates (high virulence B. xylophilus Ka4 and B. xylophilus T4, and low virulence B. xylophilus C14-5 and B. mucronatus Un1) after 24 hours exposition to H2O2.
Error bars represent standard deviation. Different letters above the columns indicate significant differences (p 2O2 neutralization, according to post-hoc Duncan's test. -
Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)H2O2 Fluorimetric Standard Curve -
Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)H2O2 Colorimetric Standard Curve
