Hydrogen Peroxide Assay Kit (Cell-based, Orange) (ab239701)
Key features and details
- Assay type: Cell-based
- Detection method: Fluorescent
- Platform: Fluorescence microscope
- Sample type: Adherent cells
Overview
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Product name
Hydrogen Peroxide Assay Kit (Cell-based, Orange)
See all Hydrogen Peroxide kits -
Detection method
Fluorescent -
Sample type
Adherent cells -
Assay type
Cell-based -
Product overview
Hydrogen Peroxide Assay Kit (Cell-based, Orange) (ab239701) uses a dye that reacts with intracellular hydrogen peroxide to produce an orange color and fluorescence, which is proportional to the concentration of intracellular hydrogen peroxide.
The kit allows for screening/studying/characterizing stimulators/inhibitors that affect intracellular levels of hydrogen peroxide.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 200 tests Assay Buffer 1 x 100ml Staining Dye Blue 1 x 10µl -
Research areas
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Relevance
Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome and immune system diseases. -
Alternative names
- H2O2
Images
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Hydrogen Peroxide staining of Macrophage cells (J774A.1).
Macrophage cells were cultured overnight and next day treated with LPS (10 mg/ml) or vehicle control for 24 hrs in the presence of staining dye. Light and fluorescence images of cells were taken using microscope. (a) and (b) are control cells, and (c) and (d) are cells treated with LPS (10 mg/ml). Treatment with LPS caused increased production of intracellular hydrogen peroxide in cells, which is demonstrated by increase fluorescent signal.
