Anti-SQSTM1 / p62 antibody (ab233207)
Key features and details
- Rabbit polyclonal to SQSTM1 / p62
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SQSTM1 / p62 antibody
See all SQSTM1 / p62 primary antibodies -
Description
Rabbit polyclonal to SQSTM1 / p62 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Rat
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Immunogen
Recombinant fragment (His-tag) corresponding to Human SQSTM1/ p62 aa 100-400. (Expressed in E.coli).
Database link: Q13501 -
Positive control
- IHC-P: Human liver, prostate gland, stomach, stomach cancer, breast cancer and skin cancer tissue. WB: HeLa, HepG2, and MCF7 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab233207)
Formalin-fixed, paraffin-embedded human skin cancer tissue stained for SQSTM1 / p62 using ab233207 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-SQSTM1 / p62 antibody (ab233207)All lanes : Anti-SQSTM1 / p62 antibody (ab233207) at 2 µg/ml
Lane 1 : Rat cerebrum lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Polyclonal Antibody (HRP) at 0.2 µg/ml
Predicted band size: 48 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab233207)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for SQSTM1 / p62 using ab233207 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab233207)Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for SQSTM1 / p62 using ab233207 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab233207)Formalin-fixed, paraffin-embedded human stomach tissue stained for SQSTM1 / p62 using ab233207 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab233207)Formalin-fixed, paraffin-embedded human prostate gland tissue stained for SQSTM1 / p62 using ab233207 at 10 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab233207)Formalin-fixed, paraffin-embedded human liver tissue stained for SQSTM1 / p62 using ab233207 at 10 µg/ml in immunohistochemical analysis. DAB staining.
