Anti-CDCP1 antibody (ab1377)
Key features and details
- Goat polyclonal to CDCP1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CDCP1 antibody
See all CDCP1 primary antibodies -
Description
Goat polyclonal to CDCP1 -
Host species
Goat -
Specificity
This antibody is expected to recognize isoform 1 (NP_073753.3) only. Approx 90kDa band observed in Human Colon lysates (calculated MW of 92.3kDa according to NP_073753.3). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide: PLLNTQEPMEPAE, corresponding to C terminal amino acids 824-836 of Human CDCP1.
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General notes
GenBank Accession Number - NP_073753
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CDCP1 antibody (ab1377)ab1377 (1 µg/ml) staining CDCP1 human colon whole cell lysate (35 µg) in RIPA buffer. Incubation was for 1 hour and detection was by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-CDCP1 antibody (ab1377)ICC/IF image of ab1377 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1377, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
