Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23162-102] to CDCP1 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free
    See all CDCP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23162-102] to CDCP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: PC-3, MDA-MB-231, MCF7 and Human colon cancer lysates. IHC-P: Human colon, Human colon cancer and Human breast cancer tissues. ICC/IF: HCT 116 cells. Flow Cyt: MCF7 cells. IP: PC-3 cells.

  • General notes

    ab264472 is the carrier-free version of ab252947. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab264472 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)
    Immunoprecipitation - Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)

    CDCP1 was immunoprecipitated from 0.35 mg PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate with ab252947 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252947 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: PC-3 whole cell lysate 10ug

    Lane 2: ab252947 IP in PC-3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252947 in PC-3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252947).

  • Flow Cytometry - Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)
    Flow Cytometry - Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell, Left) / HCT 116 (Human colorectal carcinoma epithelial cell, Right) cells labeling CDCP1 with ab252947 at 1/500 dilution (Red) compared with a Isotype control details (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody details (ab150077), at 1/2000 dilution was used as the secondary antibody.

    Negative control: MCF7 cell line (PMID: 24055141).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252947).

  • Immunocytochemistry/ Immunofluorescence - Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)
    Immunocytochemistry/ Immunofluorescence - Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HCT 116 (Human colorectal carcinoma epithelial cell) MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling CDCP1 with ab252947 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous staining in HCT 116 cell line. Negative control: MCF7 cell line (PMID: 24055141). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000  dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252947).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling CDCP1 with ab252947 at 1/200 dilution (2.8 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Mainly membranous staining in tumor cells of human breast cancer (PMID/ 16823897). The section was incubated with ab252947 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252947).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)

    Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling CDCP1 with ab252947 at 1/200 dilution (2.8 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Mainly membranous staining in tumor cells of human colon cancer (PMID: 12660814). The section was incubated with ab252947 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252947).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CDCP1 with ab252947 at 1/200 dilution (2.8 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human colon (PMID: 12660814). The section was incubated with ab252947 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252947).

  • Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)
    Anti-CDCP1 antibody [EPR23162-102] - BSA and Azide free (ab264472)

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