Anti-CDCP1 antibody [EPR23162-102] (ab252947)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23162-102] to CDCP1
- Suitable for: ICC/IF, IHC-P, WB, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-CDCP1 antibody [EPR23162-102]
See all CDCP1 primary antibodies -
Description
Rabbit monoclonal [EPR23162-102] to CDCP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP HumanWB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: PC-3, MDA-MB-231 and Human colon cancer lysates. IHC-P: Human colon, Human colon cancer and Human breast cancer tissues. ICC/IF: HCT 116 cells. Flow Cyt: MCF7 cells. IP: PC-3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23162-102 -
Isotype
IgG -
Research areas
Images
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Anti-CDCP1 antibody [EPR23162-102] (ab252947) at 1/1000 dilution + Human colon cancer lysate at 20 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 92 kDa
Observed band size: 135,70 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
The expression profile observed is consistent with what has been described in the literature (PMID: 20551327). Post-translational processing generates a C-terminal 70-kDa fragment from the full-length 135 kDa CDCP1.
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All lanes : Anti-CDCP1 antibody [EPR23162-102] (ab252947) at 1/1000 dilution
Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 135,70 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1:48 seconds;
Lanes 2-3:3 minutes.The expression profile observed is consistent with what has been described in the literature (PMID: 20551327). Post-translational processing generates a C-terminal 70-kDa fragment from the full-length 135 kDa CDCP1.
Negative control: MCF7 (PMID: 20551327, 24055141).
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CDCP1 was immunoprecipitated from 0.35 mg PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate with ab252947 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252947 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2: ab252947 IP in PC-3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252947 in PC-3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 min
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Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell, Left) / HCT 116 (Human colorectal carcinoma epithelial cell, Right) cells labeling CDCP1 with ab252947 at 1/500 dilution (Red) compared with a Isotype control details (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody details (ab150077), at 1/2000 dilution was used as the secondary antibody.
Negative control: MCF7 cell line (PMID: 24055141).
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HCT 116 (Human colorectal carcinoma epithelial cell) MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling CDCP1 with ab252947 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous staining in HCT 116 cell line. Negative control: MCF7 cell line (PMID: 24055141). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling CDCP1 with ab252947 at 1/200 dilution (2.8 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Mainly membranous staining in tumor cells of human breast cancer (PMID: 16823897). The section was incubated with ab252947 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling CDCP1 with ab252947 at 1/200 dilution (2.8 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Mainly membranous staining in tumor cells of human colon cancer (PMID: 12660814). The section was incubated with ab252947 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CDCP1 with ab252947 at 1/200 dilution (2.8 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human colon (PMID: 12660814). The section was incubated with ab252947 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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