Anti-CCM2 antibody (ab129386)
Key features and details
- Rabbit polyclonal to CCM2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CCM2 antibody
See all CCM2 primary antibodies -
Description
Rabbit polyclonal to CCM2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Recombinant full length protein (His-tag) corresponding to Human CCM2 aa 1-444.
Database link: Q9BSQ5 -
Positive control
- ICC/IF: Human foreskin tissue
WB: CCM2 overexpressed in C0S1 cells
- ICC/IF: Human foreskin tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Endotoxin level:Clonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanApplication Abreviews Notes WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 49 kDa.ICC/IF 1/200.Notes WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 49 kDa.ICC/IF
1/200.Target
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Function
May function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3-dependent p38 activation induced by hyperosmotic shock. -
Involvement in disease
Defects in CCM2 are the cause of cerebral cavernous malformations type 2 (CCM2) [MIM:603284]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and are usually present clinically during the 3rd to 5th decades of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters. -
Sequence similarities
Contains 1 PID domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 83605 Human
- Omim: 607929 Human
- SwissProt: Q9BSQ5 Human
- Unigene: 148272 Human
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Alternative names
- C7orf22 antibody
- Ccm2 antibody
- CCM2 gene antibody
see all
Images
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Western blot - Anti-CCM2 antibody (ab129386)All lanes : Anti-CCM2 antibody (ab129386) at 5 µg/ml
Lane 1 : Mwt marker
Lane 2 : CCM2 knockout
Lane 3 : CCM2 wildtype
Lane 4 : CCM2 overexpressed in C0S1 cells
Predicted band size: 49 kDa -
Western blot - Anti-CCM2 antibody (ab129386)All lanes : Anti-CCM2 antibody (ab129386) at 5 µg/ml
Lane 1 : Mwt marker
Lane 2 : CCM2 knockout
Lane 3 : CCM2 wildtype
Lane 4 : CCM2 overexpressed in C0S1 cells
Predicted band size: 49 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-CCM2 antibody (ab129386)ab129386, at a 1/50 dilution, staining CCM2 (green) in a cryo-section of unfixed Human foreskin tissue by Immunofluorescence.
A) Note specific staining in the wall of microvessels.
B) Negative control of a consecutive section.
Note non-specific fluorescence in elastic fibres in the adventitia of an arteriol.
Nuclei counter-stained with Dapi (blue).
Protocols
Datasheets and documents
References (0)
ab129386 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-CCM2 antibody (ab129386)All lanes : Anti-CCM2 antibody (ab129386) at 5 µg/ml
Lane 1 : Mwt marker
Lane 2 : CCM2 knockout
Lane 3 : CCM2 wildtype
Lane 4 : CCM2 overexpressed in C0S1 cells
Predicted band size: 49 kDa
-
Western blot - Anti-CCM2 antibody (ab129386)All lanes : Anti-CCM2 antibody (ab129386) at 5 µg/ml
Lane 1 : Mwt marker
Lane 2 : CCM2 knockout
Lane 3 : CCM2 wildtype
Lane 4 : CCM2 overexpressed in C0S1 cells
Predicted band size: 49 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-CCM2 antibody (ab129386)ab129386, at a 1/50 dilution, staining CCM2 (green) in a cryo-section of unfixed Human foreskin tissue by Immunofluorescence.
A) Note specific staining in the wall of microvessels.
B) Negative control of a consecutive section.
Note non-specific fluorescence in elastic fibres in the adventitia of an arteriol.
Nuclei counter-stained with Dapi (blue).
