Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BEND3 antibody [EPR21038-60] (ab215708)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21038-60] to BEND3
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-BEND3 antibody [EPR21038-60]
    See all BEND3 primary antibodies

  • Description

    Rabbit monoclonal [EPR21038-60] to BEND3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with BEND3(WT) expression vector containing a GFP-His-tag®, whole cell lysate; HepG2, HeLa, HEK-293T, F9, U-2 OS and NCCIT whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21038-60

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-BEND3 antibody [EPR21038-60] (ab215708)
    Western blot - Anti-BEND3 antibody [EPR21038-60] (ab215708)
    All lanes : Anti-BEND3 antibody [EPR21038-60] (ab215708) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 4 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate
    Lane 5 : U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate
    Lane 6 : NCCIT (human pluripotent embryonic carcinoma epithelial cell), whole cell lysate
    Lane 7 : HeLa treated with 10 uM MG-132 for 4 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 94 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    50 kDa unknown bands are observed.

    Exposure times: Lanes 1-3: 158 seconds; Lanes 4-8: 180 seconds.

  • Western blot - Anti-BEND3 antibody [EPR21038-60] (ab215708)
    Western blot - Anti-BEND3 antibody [EPR21038-60] (ab215708)
    All lanes : Anti-BEND3 antibody [EPR21038-60] (ab215708) at 1/5000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a GFP-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with BEND3(WT) expression vector containing a GFP-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 94 kDa
    Observed band size: 125 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 6 seconds.

  • Anti-BEND3 antibody [EPR21038-60] (ab215708)
    Anti-BEND3 antibody [EPR21038-60] (ab215708)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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