Anti-Histone H1x antibody (ab17729)
Key features and details
- Rabbit polyclonal to Histone H1x
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H1x antibody
See all Histone H1x primary antibodies -
Description
Rabbit polyclonal to Histone H1x -
Host species
Rabbit -
Specificity
ab17729 recognises Histone H1x variant in HeLa nuclear and whole cell extracts (see figures below). -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
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Positive control
- HeLa whole cell lysate and HeLa nuclear lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications MouseApplication Abreviews Notes WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).ICC/IF Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).ICC/IF
Use a concentration of 1 µg/ml.Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. -
Tissue specificity
Expressed ubiquitously. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 8971 Human
- Entrez Gene: 243529 Mouse
- Omim: 602785 Human
- SwissProt: Q92522 Human
- Unigene: 75307 Human
- Unigene: 33796 Mouse
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Alternative names
- H1 histone family member X antibody
- H1FX antibody
- H1X antibody
see all
Images
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Western blot - Anti-Histone H1x antibody (ab17729)All lanes : Anti-Histone H1x antibody (ab17729) at 0.5 µg/ml
Lane 1 : HeLa whole cell extract
Lane 2 : HeLa whole cell extract with Human Histone H1x peptide (ab18053) at 1 µg/ml
Lane 3 : HeLa nuclear extract
Predicted band size: 35 kDa
Observed band size: 35 kDa
ab17729 recognises histone H1.X in HeLa whole cell (lane1) and nuclear extracts (lane3) at 35 kDa, which is efficiently blocked using the immunizing peptide ab18053 (lane2). ab17729 recognises bands at 20 kDa, which is attributed to minor cross-reactivity and may be improved using different blocking conditions. -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1x antibody (ab17729)ICC/IF image of ab17729 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab17729, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
Datasheets and documents
References (1)
ab17729 has been referenced in 1 publication.
- Crawford NP et al. The metastasis efficiency modifier ribosomal RNA processing 1 homolog B (RRP1B) is a chromatin-associated factor. J Biol Chem 284:28660-73 (2009). WB, ICC/IF ; Human . PubMed: 19710015
Images
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Western blot - Anti-Histone H1x antibody (ab17729)All lanes : Anti-Histone H1x antibody (ab17729) at 0.5 µg/ml
Lane 1 : HeLa whole cell extract
Lane 2 : HeLa whole cell extract with Human Histone H1x peptide (ab18053) at 1 µg/ml
Lane 3 : HeLa nuclear extract
Predicted band size: 35 kDa
Observed band size: 35 kDa
ab17729 recognises histone H1.X in HeLa whole cell (lane1) and nuclear extracts (lane3) at 35 kDa, which is efficiently blocked using the immunizing peptide ab18053 (lane2). ab17729 recognises bands at 20 kDa, which is attributed to minor cross-reactivity and may be improved using different blocking conditions. -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1x antibody (ab17729)ICC/IF image of ab17729 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab17729, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
