Anti-YKL-40/CHI3L1 antibody (ab180569)
Key features and details
- Rabbit polyclonal to YKL-40/CHI3L1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-YKL-40/CHI3L1 antibody
See all YKL-40/CHI3L1 primary antibodies -
Description
Rabbit polyclonal to YKL-40/CHI3L1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Pig, Orangutan
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Immunogen
Recombinant full length protein within Human YKL-40/CHI3L1 aa 22-240. The exact sequence is proprietary.
Sequence:MGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPDALDRFL CTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWN FGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHF TTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDF ISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAP ASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEIC DFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAM VWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT
Database link: P36222 -
Positive control
- WB: MCF-7 and HT-29 cell lysates; IHC: Mouse spleen and liver tissues; ICC/ IF: Raw264.7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody (ab180569)
Paraffin-embedded mouse spleen tissue stained for YKL-40 using ab180569 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-YKL-40/CHI3L1 antibody (ab180569)Immunofluorescence staining of Raw264.7 cells stained for YKL-40 with ab180569 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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Western blot - Anti-YKL-40/CHI3L1 antibody (ab180569)All lanes : Anti-YKL-40/CHI3L1 antibody (ab180569) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : HT-29 cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat AntiRabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 43 kDa
Exposure time: 30 secondsBlocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YKL-40/CHI3L1 antibody (ab180569)Paraffin-embedded mouse liver tissue stained for YKL-40 using ab180569 at 1/100 dilution in immunohistochemical analysis.
