Human DPT Antibody Pair - BSA and Azide free (TRAMP) (ab253341)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
-
Product name
Human DPT Antibody Pair - BSA and Azide free (TRAMP)
See all DPT/TRAMP kits -
Assay type
ELISA set -
Range
18.75 pg/ml - 1200 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human DPT (TRAMP). BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab272206), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
-
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human DPT Capture Antibody (unconjugated) 1 x 100µg Human DPT Detector Antibody (unconjugated) 1 x 100µg -
Research areas
-
Function
Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation. -
Tissue specificity
Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts. -
Sequence similarities
Belongs to the dermatopontin family. -
Post-translational
modificationsSulfated on tyrosine residue(s). -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
-
Alternative names
- DERM_HUMAN
- Dermatopontin
- Dpt
see all -
Database links
- Entrez Gene: 1805 Human
- Omim: 125597 Human
- SwissProt: Q07507 Human
- Unigene: 80552 Human
Images
-
Sandwich ELISA - Human DPT Antibody Pair - BSA and Azide free (TRAMP) (ab253341)To learn more about the advantages of recombinant antibodies see here.
