Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human DPT ELISA Kit (TRAMP) (ab272206)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 2.94 pg/ml
  • Range: 18.75 pg/ml - 1200 pg/ml
  • Sample type: Cell culture media, Cit plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human DPT ELISA Kit (TRAMP)
    See all DPT/TRAMP kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 3.2%

  • Sample type

    Serum, Cell culture media, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    2.94 pg/ml

  • Range

    18.75 pg/ml - 1200 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 107 % - %
    Cell culture media 100 % - %
    Cit plasma 102 % - %

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human DPT ELISA kit (ab272206) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DPT protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DPT with 2.94 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less


            -High sensitivity, specificity and reproducibility from superior antibodies


            -Fully validated in biological samples


            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    DPT (TRAMP or Dermatopontin) is a matrix protein found in the intracellular junction of the extracellular matrix. DPT is increased in myocardial infarction and decreased in fibrosis and leiomyoma. DPT is a biomarker for giant-cell tumor of the bone and oral squamous cell carcinoma.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human DPT (TRAMP) Capture Antibody 1 x 600µl
    10X Human DPT (TRAMP) Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Human DPT (TRAMP) Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation.

  • Tissue specificity

    Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts.

  • Sequence similarities

    Belongs to the dermatopontin family.

  • Post-translational
    modifications

    Sulfated on tyrosine residue(s).

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Alternative names

    • DERM_HUMAN
    • Dermatopontin
    • Dpt
    • TRAMP
    • Tyrosine rich acidic matrix protein
    • Tyrosine-rich acidic matrix protein
    see all

  • Database links

    Images

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • Example of human DPT standard curve in Sample Diluent NS.
      Example of human DPT standard curve in Sample Diluent NS.

      The DPT standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native DPT in human serum and plasma (citrate) samples.
      Interpolated concentrations of native DPT in human serum and plasma (citrate) samples.

      The concentrations of DPT were measured in duplicates, interpolated from the DPT standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 100 and plasma (citrate) 1: 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DPT concentration was determined to be 53.79 ng/mL in serum and 63.04 ng/mL in plasma (citrate).

    • Sandwich ELISA - Human DPT ELISA Kit (TRAMP) (ab272206)
      Sandwich ELISA - Human DPT ELISA Kit (TRAMP) (ab272206)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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