Human DPT ELISA Kit (TRAMP) (ab272206)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.94 pg/ml
- Range: 18.75 pg/ml - 1200 pg/ml
- Sample type: Cell culture media, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human DPT ELISA Kit (TRAMP)
See all DPT/TRAMP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.2% -
Sample type
Serum, Cell culture media, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.94 pg/ml -
Range
18.75 pg/ml - 1200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 107 % - % Cell culture media 100 % - % Cit plasma 102 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human DPT ELISA kit (ab272206) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DPT protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DPT with 2.94 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
DPT (TRAMP or Dermatopontin) is a matrix protein found in the intracellular junction of the extracellular matrix. DPT is increased in myocardial infarction and decreased in fibrosis and leiomyoma. DPT is a biomarker for giant-cell tumor of the bone and oral squamous cell carcinoma.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human DPT (TRAMP) Capture Antibody 1 x 600µl 10X Human DPT (TRAMP) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human DPT (TRAMP) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation. -
Tissue specificity
Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts. -
Sequence similarities
Belongs to the dermatopontin family. -
Post-translational
modificationsSulfated on tyrosine residue(s). -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- DERM_HUMAN
- Dermatopontin
- Dpt
see all -
Database links
- Entrez Gene: 1805 Human
- Omim: 125597 Human
- SwissProt: Q07507 Human
- Unigene: 80552 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human DPT standard curve in Sample Diluent NS.The DPT standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native DPT in human serum and plasma (citrate) samples.The concentrations of DPT were measured in duplicates, interpolated from the DPT standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 100 and plasma (citrate) 1: 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DPT concentration was determined to be 53.79 ng/mL in serum and 63.04 ng/mL in plasma (citrate).
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Sandwich ELISA - Human DPT ELISA Kit (TRAMP) (ab272206)To learn more about the advantages of recombinant antibodies see here.
