Anti-POLD1 antibody (ab225907)
Key features and details
- Rabbit polyclonal to POLD1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-POLD1 antibody
See all POLD1 primary antibodies -
Description
Rabbit polyclonal to POLD1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human POLD1 aa 850-900. The exact sequence is proprietary. (NP_002682.2).
Database link: P28340 -
Positive control
- WB: HeLa, HEK-293T, Jurkat and TCMK-1 whole cell lysates. IP: HeLa whole cell lysate.
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General notes
This product was previously labelled as DNA Polymerase delta
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab225907 was affinity purified using an epitope specific to POLD1, catalytic subunit immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-POLD1 antibody (ab225907)All lanes : Anti-POLD1 antibody (ab225907) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-POLD1 antibody (ab225907)POLD1, catalytic subunit was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225907 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225907 at 1 µg/ml.
Lane 1: ab225907 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
