Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (ab232609)
![Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (ab232609)](https://www.abcam.com/ps/products/232/ab232609/Images/ab232609-308806-2326091.jpg "Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (ab232609)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13832] to POLDIP2 - BSA and Azide free
- Suitable for: WB, Flow Cyt, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free
See all POLDIP2 primary antibodies -
Description
Rabbit monoclonal [EPR13832] to POLDIP2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1 whole cell lysate. HeLa and HepG2 whole cell lysate.
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General notes
Ab232609 is the carrier-free version of ab181841. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232609 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13832 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (ab232609)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: POLDIP2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab181841 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.ab181841 was shown to specifically recognize POLDIP2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when POLDIP2 knockout samples were examined. Wild-type and POLDIP2 knockout samples were subjected to SDS-PAGE. ab181841 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1,000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181841).
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![Flow Cytometry - Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (ab232609)](https://www.abcam.com/ps/products/232/ab232609/Images/ab232609-3-ab181841212752ab181841FC.jpg)
Flow Cytometry - Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (ab232609)Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling POLDIP2 with ab181841 at 1/350 diltuion (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181841).
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![Immunoprecipitation - Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (ab232609)](https://www.abcam.com/ps/products/232/ab232609/Images/ab232609-2-ab181841212750ab181841.jpg)
Immunoprecipitation - Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (ab232609)Western blot analysis of HeLa cell lysate immunoprecipitated with ab181841 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181841).
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![Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (ab232609)](https://www.abcam.com/ps/products/232/ab232609/Images/ab232609-4-benefits-of-recombinant-antibodies.png)
Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (ab232609)
