Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLBP antibody [EPR12673] - BSA and Azide free (ab238997)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12673] to SLBP - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-SLBP antibody [EPR12673] - BSA and Azide free
    See all SLBP primary antibodies

  • Description

    Rabbit monoclonal [EPR12673] to SLBP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: MCF7 cells.

  • General notes

    Ab238997 is the carrier-free version of ab181972. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238997 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12673

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-SLBP antibody [EPR12673] - BSA and Azide free (ab238997)
    Immunoprecipitation - Anti-SLBP antibody [EPR12673] - BSA and Azide free (ab238997)

    ab181972 (purified) at 1:20 dilution (1µ) immunoprecipitating SLBP in 293T whole cell lysate.
    Lane 1 (input): 293T (Human embryonic kidney epithelial cell) whole cell lysate 10µ
    Lane 2 (+): ab181972 & 293T whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181972 in 293T whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181972).

  • Flow Cytometry - Anti-SLBP antibody [EPR12673] - BSA and Azide free (ab238997)
    Flow Cytometry - Anti-SLBP antibody [EPR12673] - BSA and Azide free (ab238997)

    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling SLBP with Purified ab181972 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181972).

  • Flow Cytometry - Anti-SLBP antibody [EPR12673] - BSA and Azide free (ab238997)
    Flow Cytometry - Anti-SLBP antibody [EPR12673] - BSA and Azide free (ab238997)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling SLBP with ab181972 at 1/30 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (greeen), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181972).

  • Immunoprecipitation - Anti-SLBP antibody [EPR12673] - BSA and Azide free (ab238997)
    Immunoprecipitation - Anti-SLBP antibody [EPR12673] - BSA and Azide free (ab238997)

    Western blot analysis of Jurkat cell lysate immunoprecipitated with ab181972 at 1/50 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG used at 1/1500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181972).

  • Anti-SLBP antibody [EPR12673] - BSA and Azide free (ab238997)
    Anti-SLBP antibody [EPR12673] - BSA and Azide free (ab238997)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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