Mouse Erythropoietin ELISA Kit (EPO) (ab270893)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.35 pg/ml
- Range: 9.375 pg/ml - 600 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Erythropoietin ELISA Kit (EPO)
See all EPO kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.2% Inter-assay Sample n Mean SD CV% Serum 3 7.5% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.35 pg/ml -
Range
9.375 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105 99% - 112% Cell culture media 100 99% - 101% Hep Plasma 97 89% - 104% EDTA Plasma 99 98% - 101% Cit plasma 95 91% - 98% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Erythropoietin ELISA kit (ab270893) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Erythropoietin protein in mouse serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate mouse Erythropoietin with 2.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Erythropoietin is the principal hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Erythropoietin binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. Erythropoietin is a secreted protein produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. Human erythropoietin is used for the treatment of anemia. Variations affecting the human EPO gene are associated with microvascular complications of diabetes 2. Mouse Erythropoietin has 94% amino acids sequence identity to rat Erythropoietin, and 80% identity to the human Erythropoietin.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Erythropoietin (EPO) Capture Antibody 1 x 600µl 10X Mouse Erythropoietin (EPO) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse Erythropoietin (EPO) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. -
Cellular localization
Secreted -
Alternative names
- EP
- EPO
- EPO alpha
see all -
Database links
- Entrez Gene: 13856 Mouse
- SwissProt: P07321 Mouse
- Unigene: 349116 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse Erythropoietin competitor curve comparisonStandard Curve comparison between Mouse Erythropoiethin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of mouse Erythropoietin standard curve in Sample Diluent NS.The Erythropoietin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Erythropoietin in mouse CD-1 plasma samples.The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 100%, plasma (EDTA) 100% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 95 pg/mL in plasma (citrate), 36 pg/mL in plasma (EDTA) and 153 pg/mL in plasma (heparin).
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Interpolated concentrations of native Erythropoietin in mouse C57BL/6, CD-1 and Balb/C serum samples.The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: C57BL/6 serum 100%, CD-1 serum 100% and Balb/C serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 478 pg/mL in C57BL/6 serum, 66 pg/mL in CD-1 serum and 68 pg/mL in Balb/C serum.
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Sandwich ELISA - Mouse Erythropoietin ELISA Kit (EPO) (ab270893)To learn more about the advantages of recombinant antibodies see here.
