Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLBP antibody [EPR12673] (ab181972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12673] to SLBP
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-SLBP antibody [EPR12673]
    See all SLBP primary antibodies

  • Description

    Rabbit monoclonal [EPR12673] to SLBP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, 293T and Jurkat whole cell lysate (ab7899); HeLa cells; Flow Cyt: MCF7 and Jurkat cells. IP: 293T

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12673

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SLBP antibody [EPR12673] (ab181972)
    Western blot - Anti-SLBP antibody [EPR12673] (ab181972)
    Anti-SLBP antibody [EPR12673] (ab181972) at 1/5000 dilution + 293T (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 31 kDa

  • Flow Cytometry - Anti-SLBP antibody [EPR12673] (ab181972)
    Flow Cytometry - Anti-SLBP antibody [EPR12673] (ab181972)

    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling SLBP with Purified ab181972 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-SLBP antibody [EPR12673] (ab181972)
    Immunoprecipitation - Anti-SLBP antibody [EPR12673] (ab181972)

    ab181972 (purified) at 1:20 dilution (1µg) immunoprecipitating SLBP in 293T whole cell lysate.
    Lane 1 (input): 293T (Human embryonic kidney epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab181972 & 293T whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181972 in 293T whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-SLBP antibody [EPR12673] (ab181972)
    Western blot - Anti-SLBP antibody [EPR12673] (ab181972)
    All lanes : Anti-SLBP antibody [EPR12673] (ab181972) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 31 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-SLBP antibody [EPR12673] (ab181972)
    Flow Cytometry - Anti-SLBP antibody [EPR12673] (ab181972)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling SLBP with ab181972 at 1/30 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (greeen), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunoprecipitation - Anti-SLBP antibody [EPR12673] (ab181972)
    Immunoprecipitation - Anti-SLBP antibody [EPR12673] (ab181972)

    Western blot analysis of Jurkat cell lysate immunoprecipitated with ab181972 at 1/50 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG used at 1/1500 dilution.

  • Anti-SLBP antibody [EPR12673] (ab181972)
    Anti-SLBP antibody [EPR12673] (ab181972)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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