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Neuroscience Sensory System Auditory system

Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408)

Price and availability

321 638 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20867-248] to cleaved N-terminal DFNA5 / GSDME
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248]
    See all cleaved N-terminal DFNA5 / GSDME primary antibodies
  • Description

    Rabbit monoclonal [EPR20867-248] to cleaved N-terminal DFNA5 / GSDME
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SH-SY5Y (treated with etoposide (60uM, 14h) whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20867-248
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Sensory System
    • Auditory system

Images

  • Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408)
    Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408)
    All lanes : Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408) at 1/1000 dilution

    Lane 1 : Wild-type SH-SY5Y (human neuroblastoma cell line from bone marrow) (treated with treated with etoposide (60uM, 14h), whole cell lysate
    Lane 2 : Untreated wild-type SH-SY5Y whole cell lysate
    Lane 3 : DFNA5/GSDME knockout SH-SY5Y treated with etoposide (60 uM, 14h), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?


    Exposure time: 27 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    Lysates were kindly provided by Dr Feng Shao.

    The molecular weight observed is consistent with the literature (PMID: 28459430, 30804337).

  • Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408)
    Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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