Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11071] to Emerin - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Emerin antibody [EPR11071] - BSA and Azide free
    See all Emerin primary antibodies

  • Description

    Rabbit monoclonal [EPR11071] to Emerin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HeLa, and HEK-293T whole cell lysates; IHC-P: Human breast, skeletal muscle thyroid gland carcinoma tissues; ICC/IF: HeLa cells; Flow Cyt: HeLa cells.

  • General notes

    Ab240138 is the carrier-free version of ab156871. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240138 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)
    Western blot - Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)
    All lanes : Anti-Emerin antibody [EPR11071] (ab156871) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : EMD knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab156871).

    Lanes 1-2: Merged signal (red and green). Green - ab156871 observed at 35 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab156871 Anti-Emerin antibody [EPR11071] was shown to specifically react with Emerin in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266336 (knockout cell lysate ab257423) was used. Wild-type and Emerin knockout samples were subjected to SDS-PAGE. ab156871 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)
    Immunocytochemistry/ Immunofluorescence - Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Emerin with Purified ab156871 at 1:50 dilution (6.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156871).

  • Flow Cytometry - Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)
    Flow Cytometry - Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Emerin with Purified ab156871 at 1:30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156871).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)

    Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling Emerin with unpurified ab156871 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156871).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Western blot - Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)
    Western blot - Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)
    All lanes : Anti-Emerin antibody [EPR11071] (ab156871) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    Blocking/Diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156871).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Emerin with unpurified ab156871 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156871).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue sections labeling Emerin with purified ab156871 at 1/500 dilution (0.652 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156871).

  • Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)
    Anti-Emerin antibody [EPR11071] - BSA and Azide free (ab240138)

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