Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15656] to Androgen Receptor (AR-V7 specific) - Low endotoxin, Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free
    See all Androgen Receptor (AR-V7 specific) primary antibodies

  • Description

    Rabbit monoclonal [EPR15656] to Androgen Receptor (AR-V7 specific) - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Specificity

    This target is a splice variant of AR lacking the C-terminal androgen binding site.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 22Rv1 whole cell lysate. IHC-P: Human prostate cancer tissue.

  • General notes

    ab209491 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    For Androgen receptor variant 5,6,7es (ARv567es) specific RabMAb product - see ab200827.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15656

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)

    Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nucleus staining on cancer cells of Human prostate cancer (case#:F123132) is observed.

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    PLoS One. 2011;6(11):e27970. PMID:22114732

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)

    Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.

    Negative on cancer cells of Human prostate cancer (case#:F11194)

     

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    PLoS One. 2011;6(11):e27970. PMID:22114732

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)

    Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.

    Negative on cancer cells of Human prostate cancer (case#:F121524)

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    PLoS One. 2011;6(11):e27970. PMID:22114732

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.

    Negative staining on normal Human liver tissue.

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling AR-V7 with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.

    Negative staining on Human hepatocellular carcinoma.

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)
    Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)
    Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491) + 22RV1 (Human prostate carcinoma cell line) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 68 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM/TBST

  • Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)
    Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)

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