Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

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Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15656] to Androgen Receptor (AR-V7 specific)
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656]
See all Androgen Receptor (AR-V7 specific) primary antibodies
Description

Rabbit monoclonal [EPR15656] to Androgen Receptor (AR-V7 specific)
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
(Peptide available as ab223099)
Positive control
- WB: 22Rv1 whole cell lysate. IHC-P: Human prostate cancer and breast tumor tissue.
General notes
Note that for IHC it is recommended to use high pH (9.0) antigen retrieval buffer and a high sensitivity detection system, such as our Rabbit Specific IHC polymer detection kit HRP/DAB (ab209101).

For Androgen receptor variant 5,6,7es (ARv567es) specific RabMAb product - see ab200827.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15656
Isotype

IgG

Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

All lanes : Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394) at 1/1000 dilution

Lane 1 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg
Lane 2 : PC-3 (Human prostate cancer cell line) whole cell lysate at 10 µg
Lane 3 : LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg
Lane 4 : Human fetal liver lysate at 10 µg
Lane 5 : Human prostate lysate at 10 µg
Lane 6 : 22Rv1 (Human prostate carcinoma cell line) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 80 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on cancer cells of human prostate cancer (case#:F123132) is observed.

Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

PLoS One. 2011;6(11):e27970. PMID:22114732

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394) Image from Hickey et al., Oncotarget., 6(42): 44728–44744. Fig 2 (Panel D).; doi: https://doi.org/10.18632/oncotarget.6296. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/3.0/.

Detection of AR-V7 protein by IHC in 3 breast tumors with high levels of AR-V7 mRNA expression (left). Positive staining was not detected in specimens that do not express AR-V7 mRNA (a representative is shown on the right). Inset images of higher magnification demonstrate strong nuclear staining. Refer to PMID 26554309.

Image from Hickey et al., Oncotarget., 6(42): 44728–44744. Fig 2 (Panel D).; doi: https://doi.org/10.18632/oncotarget.6296

license: https://creativecommons.org/licenses/by/3.0/
Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

All lanes : Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394) at 1/1000 dilution (Purified)

Lane 1 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method
Lane 2 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol here (downloadable copy).

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

Negative on cancer cells of human prostate cancer (case#:F11194)

Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

PLoS One. 2011;6(11):e27970. PMID:22114732

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

Negative on cancer cells of human prostate cancer (case#:F121524)

Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

PLoS One. 2011;6(11):e27970. PMID:22114732

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

Negative staining on normal human liver tissue.

Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling AR-V7 with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

Negative staining on human hepatocellular carcinoma.

Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

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