Anti-Phospho SQ/TQ ATM/ATR Substrate antibody (ab130947)
Key features and details
- Rabbit polyclonal to Phospho SQ/TQ ATM/ATR Substrate
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Phospho SQ/TQ ATM/ATR Substrate antibody -
Description
Rabbit polyclonal to Phospho SQ/TQ ATM/ATR Substrate -
Host species
Rabbit -
Specificity
Ab130947 recognizes proteins phosphorylated on SQ/TQ motifs (ATM/ATR kinase consensus phosphorylation site motif) -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Phospho SQ/TQ ATM/ATR Substrate aa 2600-2700 (phospho S2609 + T2609) conjugated to keyhole limpet haemocyanin.
Database link: P78527 -
Positive control
- This antibody gave a positive signal in Western Blot in the following cell line: HeLa untreated and UV treated whole cell
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 450 kDa (predicted molecular weight: 469 kDa).An increase in signal is observed upon UV treatment
Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 450 kDa (predicted molecular weight: 469 kDa).An increase in signal is observed upon UV treatment
Target
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Relevance
Several protein kinases mediate cellular responses to DNA damage. This includes the serine/threonine kinases ATM (ataxia telangiectasia mutated) and ATR (ATM and Rad3-related). In response to DNA damage ATM and ATR phosphorylate a large range of proteins on Serine (S) or Threonine (T) residues next to a Glutamine (Q) residue, so called SQ or TQ consensus sites. -
Alternative names
- ATM antibody
- ATR antibody
- DNA PK antibody
see all
Images
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Western blot - Anti-Phospho SQ/TQ ATM/ATR Substrate antibody (ab130947)All lanes : Anti-Phospho SQ/TQ ATM/ATR Substrate antibody (ab130947) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Hela Whole Cell Lysate - UV Irradiated
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 469 kDa
Observed band size: 450 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab130947 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (2)
ab130947 has been referenced in 2 publications.
- Cao L et al. Genotoxic stress-triggered ß-catenin/JDP2/PRMT5 complex facilitates reestablishing glutathione homeostasis. Nat Commun 10:3761 (2019). PubMed: 31434880
- Wang D et al. Colonic Lysine Homocysteinylation Induced by High-Fat Diet Suppresses DNA Damage Repair. Cell Rep 25:398-412.e6 (2018). PubMed: 30304680
Images
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Western blot - Anti-Phospho SQ/TQ ATM/ATR Substrate antibody (ab130947)All lanes : Anti-Phospho SQ/TQ ATM/ATR Substrate antibody (ab130947) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Hela Whole Cell Lysate - UV Irradiated
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 469 kDa
Observed band size: 450 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab130947 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.