4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for GABRE (green, C) using ab241998 at 1/100 dilution for 1 hour at room temperature in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100 dilution. Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a composite image.

Anti-GABRE antibody [S413-67] (ab241998) at 1/1000 dilution + Rat brain tissue lysate at 10 µg

Secondary
Goat Anti-Mouse IgG: HRP at 1/200 dilution

Developed using the ECL technique.

Predicted band size: 58 kDa


Exposure time: 6 minutes

Primary and secondary incubation for 1 hour at room temperature.

Blocked for 1 hr at RT.