Anti-NPFF1 Receptor antibody (ab45350)
Key features and details
- Rabbit polyclonal to NPFF1 Receptor
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NPFF1 Receptor antibody
See all NPFF1 Receptor primary antibodies -
Description
Rabbit polyclonal to NPFF1 Receptor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatWB Human
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Immunogen
Synthetic peptide corresponding to Human NPFF1 Receptor aa 350 to the C-terminus.
(Peptide available asab45349) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: SHSY5Y.
This antibody gave a positive signal in the following cell lines: PC12.
- This antibody gave a positive signal in the following whole cell lysates: SHSY5Y.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF RatWB HumanApplication Abreviews Notes WB Use a concentration of 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 48 kDa).ICC/IF Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 48 kDa).ICC/IF
Use a concentration of 1 µg/ml.Target
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Function
Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 64106 Human
- Entrez Gene: 64107 Rat
- Omim: 607448 Human
- SwissProt: Q9GZQ6 Human
- SwissProt: Q9EP86 Rat
- Unigene: 302026 Human
- Unigene: 64483 Rat
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Alternative names
- G Protein Coupled Receptor 147 antibody
- G-protein coupled receptor 147 antibody
- GPR147 antibody
see all
Images
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Western blot - Anti-NPFF1 Receptor antibody (ab45350)
The peptide blocking data above shows that ab45350 detects a protein of approximately 64 kDa, thought to be the NPFF1 Receptor. It is possible that this band is higher than expected due to modifications at potential glycosylation sites.
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Immunocytochemistry/ Immunofluorescence - Anti-NPFF1 Receptor antibody (ab45350)ICC/IF image of ab45350 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45350, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (0)
ab45350 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-NPFF1 Receptor antibody (ab45350)
The peptide blocking data above shows that ab45350 detects a protein of approximately 64 kDa, thought to be the NPFF1 Receptor. It is possible that this band is higher than expected due to modifications at potential glycosylation sites.
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Immunocytochemistry/ Immunofluorescence - Anti-NPFF1 Receptor antibody (ab45350)
ICC/IF image of ab45350 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45350, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
