Magnetic Antibody Purification System (ab270532)
Overview
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Product name
Magnetic Antibody Purification System -
Product overview
Magnetic Antibody Purification System (ab270532) is a convenient method to purify antibodies at a small scale with high recoveries. It quickly removes substances such as BSA, glycine, tris and azide that interfere with conjugation reactions. It can be easily adapted to your needs allowing you to purify from 20 to 200 μg of antibody and to reach the desired final antibody concentration. The antibody to be purified or cleaned up should ideally be in a volume of 20 μl to 1 ml.
No dialysis of the antibody is needed prior to conjugation as interfering substances such as Tris, citrate and glycine have been replaced with conjugation-friendly alternatives.
This sytem can directly replace any existing protein A purification kit and can be used to isolate IgG antibodies from crude mixtures.
- Less than 15 minutes hands on time.
- 70-90% antibody recovery.
- Up to 10 purifications at the smallest working scale.
This product is manufactured by Expedeon, an Abcam company. It was previously called AbPure™ Universal Magnetic Purification System. Expedeon product code 265-0200 is the same as the 400 µL size of this product.
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Notes
This product is manufactured by Expedeon, an Abcam company. It was previously called AbPure™ Universal Magnetic Purification System. Expedeon product code 265-0200 is the same as the 400 µL size of this product.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 400 µl ab273970 - Binding Buffer (10X) 1 x 1ml ab273968 - Elution Buffer 1 x 1ml ab273971 - Neutralizaton Buffer 1 x 500µl ab273972 - Protein A Magnetic Beads 1 x 400µl ab273969 - Wash Buffer 1 x 15ml
Images
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How does the kit work?
The method involves capturing the antibody on the Protein A Magnetic Beads. Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species; once the antibody has bound to the Protein A, unwanted substances can be removed by simply collecting the beads in a magnetic stand and washing the beads. The antibody is then eluted and neutralized.
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Rabbit serum purificationA sample of rabbit serum has been purified by using Magnetic Antibody Purification System (ab270532).
The gel picture shows how all the contaminants are effectively removed and the purified rabbit IgG is eluted.
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Rabbit IgG purification20 µg (A), 25 µg (B) or 50 µg (C) rabbit IgG was purified using Magnetic Antibody Purification System (ab270532), demonstrating high recoveries in the first elution fraction even at the smallest working scale.