Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24453-121] to Thyroxine Binding Globulin - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt (Intra), IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free
    See all Thyroxine Binding Globulin primary antibodies

  • Description

    Rabbit monoclonal [EPR24453-121] to Thyroxine Binding Globulin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cyt (Intra), IHC-Pmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma, small intestine, and lung tissue lysates. IHC-P: Human colon and placenta tissues. Flow cyt: HepG2 cells. IP: Human lung tissue lysate; HepG2 whole cell lysate

  • General notes

    ab280962 is the carrier-free version of ab280954. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280962 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24453-121

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)

    This data was developed using ab280954, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Thyroxine Binding Globulin with ab280954 at 1/300 (5.92 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the plasma in human colon. The section was incubated with ab280954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
    Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)

    This data was developed using ab280954, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HepG2 (Human hepatocellular carcinoma epithelial cell, Right) cells labelling Thyroxine Binding Globulin with ab280954 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: HeLa cells.

  • Western blot - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
    Western blot - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
    All lanes : Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954) at 1/1000 dilution

    Lane 1 : Human plasma at 20 µl
    Lane 2 : Human small intestine tissue lysate at 40 µg
    Lane 3 : Human lung tissue lysate 40ug at 40 µg
    Lane 4 : Human skeletal muscle lysate at 40 µg

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
    Lanes 2-4 : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 49,54 kDa why is the actual band size different from the predicted?



    This data was developed using 280954, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Samples are non-boiled as boiling may cause protein aggregates. 

     The MW observed is consistent with the literature (PMID: 12107243).

    Thyroxine-binding globulin is highly expressed in plasma. (PMID:820495, 8106612).

    Bands of 54KDa and 49KDa respectively represent the full length and endogenous cleavage form.

     Exposure time: Lane 1: 15 seconds Lane 2: 37 seconds Lane 3, 4: 109 seconds.

     

  • Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
    Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)

    This data was developed using ab280954, the same antibody clone in a different buffer formulation.

    Thyroxine Binding Globulin was immunoprecipitated from 0.35 mg Human lung tissue lysate with ab280954 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280954 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human lung tissue lysate 10 ug

    Lane 2: ab280954 IP in Human lung tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280954 in Human lung tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This blot was developed using a higher sensitivity ECL substrate.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)

    This data was developed using ab280954, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling Thyroxine Binding Globulin with ab280954 at 1/300 (5.92 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the plasma in human placenta. The section was incubated with ab280954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
    Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)

    This data was developed using ab280954, the same antibody clone in a different buffer formulation.

    Thyroxine Binding Globulin was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) supernatant with ab280954 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280954 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) supernatant 10 µl

    Lane 2: ab280954 IP in HepG2 supernatant

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280954 in HepG2 supernatant

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)

    This data was developed using ab280954, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling Thyroxine Binding Globulin with ab280954 at 1/300 (5.92 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining on human skeletal muscle (PMID: 8143919). The section was incubated with ab280954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
    Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)

    This data was developed using ab280954, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells labelling Thyroxine Binding Globulin with ab280954 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: HEK-293T cells.

  • Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
    Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)

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