Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824)
![Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824)](https://www.abcam.com/ps/products/219/ab219824/Images/ab219824-286099-ab2198241.jpg "Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824)")
Key features and details
- Mouse monoclonal [5D11-1C9] to MTCO1 - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-MTCO1 antibody [5D11-1C9] - N-terminal -
Description
Mouse monoclonal [5D11-1C9] to MTCO1 - N-terminal -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MTCO1 (N terminal).
Database link: P00395 -
Positive control
- WB: Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn); Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn).
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General notes
Western blot advice:
Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.36% HEPES, 0.87% Sodium chloride -
Concentration information loading... -
Purification notes
Purified from hybridoma cell culture supernatant by Protein L affinity chromatography from fetal bovine serum containing medium (Protein L does not bind bovine IgG). -
Clonality
Monoclonal -
Clone number
5D11-1C9 -
Isotype
IgG2a -
Light chain type
kappa
Images
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Western blot - Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824)All lanes : Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824) at 0.1 µg/ml
Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn)
Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled Goat-anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.
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![Western blot - Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824)](https://www.abcam.com/ps/products/219/ab219824/Images/ab219824-286100-ab2198242.jpg)
Western blot - Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824)All lanes : Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824) at 2 µg/ml
Lane 1 : Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn)
Lane 2 : Whole cell extract HDFn-Rho0 cells depleted of mtDNA by long-term culture in the presence of ethidium bromide
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-labeled Goat-anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 57 kDaProteins were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.
