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Immunology Immunoglobulins Heavy Chain IgM

Anti-Human IgM antibody [EPR5539-65-4] - BSA and Azide free (ab236941)

Anti-Human IgM antibody [EPR5539-65-4] - BSA and Azide free (ab236941)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5539-65-4] to Human IgM - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Human IgM antibody [EPR5539-65-4] - BSA and Azide free
    See all Human IgM primary antibodies
  • Description

    Rabbit monoclonal [EPR5539-65-4] to Human IgM - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified).

  • Positive control

    • IHC: Human tonsil tissue. WB: Raji cells; IP: Raji whole cell lysate.
  • General notes

    ab236941 is the carrier-free version of ab134159 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab236941 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5539-65-4
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgM

Images

  • Western blot - Anti-Human IgM antibody [EPR5539-65-4] - BSA and Azide free (ab236941)
    Western blot - Anti-Human IgM antibody [EPR5539-65-4] - BSA and Azide free (ab236941)
    Anti-Human IgM antibody [EPR5539-65-4] (ab134159) at 1/1000 dilution (Purified) + Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab134159, the same antibody clone in a different buffer formulation. 

    Blocking Buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgM antibody [EPR5539-65-4] - BSA and Azide free (ab236941)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgM antibody [EPR5539-65-4] - BSA and Azide free (ab236941)

    This data was developed using ab134159, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling Human IgM with Purified ab236941 at 1:1000 dilution (0.16 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunoprecipitation - Anti-Human IgM antibody [EPR5539-65-4] - BSA and Azide free (ab236941)
    Immunoprecipitation - Anti-Human IgM antibody [EPR5539-65-4] - BSA and Azide free (ab236941)

    This data was developed using ab134159, the same antibody clone in a different buffer formulation.
    purifiedab134159 at 1/50 dilution (2µg) immunoprecipitating Human IgM in Raji whole cell lysate.
    Lane 1 (input): Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab134159 + Raji whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134159 in Raji whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 75 kDa

  • Anti-Human IgM antibody [EPR5539-65-4] - BSA and Azide free (ab236941)
    Anti-Human IgM antibody [EPR5539-65-4] - BSA and Azide free (ab236941)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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