Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Exportin-5 antibody [EPR8452] - BSA and Azide free (ab248256)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8452] to Exportin-5 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Exportin-5 antibody [EPR8452] - BSA and Azide free
    See all Exportin-5 primary antibodies

  • Description

    Rabbit monoclonal [EPR8452] to Exportin-5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248256 is the carrier-free version of ab129006. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248256 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Exportin-5 antibody [EPR8452] - BSA and Azide free (ab248256)
    Western blot - Anti-Exportin-5 antibody [EPR8452] - BSA and Azide free (ab248256)
    All lanes : Anti-Exportin-5 antibody [EPR8452] (ab129006) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Human fetal heart tissue lysate
    Lane 4 : Human fetal brain tissue lysate
    Lane 5 : C6 cell lysate
    Lane 6 : RAW 264.7 cell lysate
    Lane 7 : PC-12 cell lysate
    Lane 8 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 136 kDa
    Observed band size: 136 kDa



    This data was developed using ab129006, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-Exportin-5 antibody [EPR8452] - BSA and Azide free (ab248256)
    Flow Cytometry - Anti-Exportin-5 antibody [EPR8452] - BSA and Azide free (ab248256)
    This data was developed using ab129006, the same antibody clone in a different buffer formulation.Overlay histogram showing HEK293 cells stained with ab129006 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129006, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Exportin-5 antibody [EPR8452] - BSA and Azide free (ab248256)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Exportin-5 antibody [EPR8452] - BSA and Azide free (ab248256)
    This data was developed using ab129006, the same antibody clone in a different buffer formulation.ab129006, at 1/250 dilution, staining Exportin-5 in paraffin-embedded Human colon tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-Exportin-5 antibody [EPR8452] - BSA and Azide free (ab248256)
    OI-RD Scanning - Anti-Exportin-5 antibody [EPR8452] - BSA and Azide free (ab248256)
    This data was developed using ab129006, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Exportin-5 antibody [EPR8452] - BSA and Azide free (ab248256)
    Anti-Exportin-5 antibody [EPR8452] - BSA and Azide free (ab248256)

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