Anti-POLR3B antibody [EPR8719] (ab137030)
![Anti-POLR3B antibody [EPR8719] (ab137030)](https://www.abcam.com/ps/products/137/ab137030/Images/POLR3B-Primary-antibodies-ab137030-1.jpg "Anti-POLR3B antibody [EPR8719] (ab137030)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8719] to POLR3B
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-POLR3B antibody [EPR8719]
See all POLR3B primary antibodies -
Description
Rabbit monoclonal [EPR8719] to POLR3B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide corresponding to residues in Human POLR3B (UniProt ID: Q9NW08)
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Positive control
- Hela or HepG2 cell lysates; 293T cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Dissociation constant (KD)
KD = 2.37 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8719 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-POLR3B antibody [EPR8719] (ab137030)All lanes : Anti-POLR3B antibody [EPR8719] (ab137030) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Hela cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 128 kDa
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![Flow Cytometry - Anti-POLR3B antibody [EPR8719] (ab137030)](https://www.abcam.com/ps/products/137/ab137030/Images/POLR3B-Primary-antibodies-ab137030-2.jpg)
Flow Cytometry - Anti-POLR3B antibody [EPR8719] (ab137030)Flow cytometry analysis of permeabilized 293T cells labelling POLR3B with ab137030 at 1/10 dilution (red) or a negative rabbit IgG (green). -
![OI-RD Scanning - Anti-POLR3B antibody [EPR8719] (ab137030)](https://www.abcam.com/ps/products/137/ab137030/Images/POLR3B-Primary-antibodies-ab137030-3.jpg)
OI-RD Scanning - Anti-POLR3B antibody [EPR8719] (ab137030) -
![Anti-POLR3B antibody [EPR8719] (ab137030)](https://www.abcam.com/ps/products/137/ab137030/Images/ab137030-4-benefits-of-recombinant-antibodies.png)
Anti-POLR3B antibody [EPR8719] (ab137030)