Anti-GTF3C5 antibody (ab74305)
Key features and details
- Rabbit polyclonal to GTF3C5
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-GTF3C5 antibody -
Description
Rabbit polyclonal to GTF3C5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
-
Immunogen
Synthetic peptide from a region between residue 469 and 519 of human GTF3C5 (using the numbering given in entry NP_036219.1)
-
Positive control
- Lysate from HeLa, 293T or NIH3T3 cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF3C5 antibody (ab74305)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling GTF3C5 with ab74305 at 1/500 (0.4µg/ml). Detection: DAB.
-
Western blot - Anti-GTF3C5 antibody (ab74305)All lanes : Anti-GTF3C5 antibody (ab74305) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 60 kDa
Observed band size: 60 kDa
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
-
Immunoprecipitation - Anti-GTF3C5 antibody (ab74305)20% of immunoprecipitate obtained from 1mg HeLa whole cell lysate using 3µg ab74305 or a control IgG was loaded in lane 1 and 2 respectively. GTF3C5 was then detected by WB, using ab74305 at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
